Detecting Pseudomonas aeruginosa and Staphylococcus aureus with the MolecuLight i:X™

Most potentially harmful bacteria fluoresce red when imaged with the MolecuLight i:X (e.g. Staphylococcus aureus), however Pseudomonas aeruginosa fluoresces cyan. In this patient, MolecuLight i:X images revealed both cyan and red fluorescence, which cultures confirmed to be P. aeruginosa and S. aureus. Real-time bacterial visualization and early detection of these pathogens is vital, as polymicrobial P. aeruginosa and S. aureus infections are more virulent than single pathogen infections and result in worse patient outcomes1,2. In this case, MolecuLight i:X guidance led to sampling a region with both red and cyan signals, increasing the likelihood of detecting both species.

Case-Study-0044-Standard-Imaging-Mode   Case-Study-0044-Fluorescence-Imaging-Mode-1   Case-Study-0044-Fluorescence-Imaging-Mode-2
Standard Imaging ModeTM   Fluorescence Imaging ModeTM
Cyan color* (arrows) is indicative of P. aeruginosa and blush red (circled) was confirmed to be Staphylococcus aureus

Wound Etiology
  • Large diabetic foot ulcer
Anatomical Location
  • Left heel
Confirmed Lab Pathologies
  • Curettage samples revealed heavy growth of Pseudomonas aeruginosa and light growth of Staphylococcus aureus
Images provided by Dr. Ron Linden, Judy Dan Wound Care Centre, ON, Canada
MolecuLight Clinical Case 0044.

* Fluorescence signal oversaturation from heavily concentrated P. aeruginosa may appear more white than cyan.
1 Pastar I et al. 2013. PLoS One 8:e56846
2 Hendricks KJ et al. 2001. J. Bone Joint Surg. Am. 83-A:855–861.